Microbiology QC Plan

for your Wine Fill Line

Stage	Sampling Location	Test for	Method	DETECTION ORDERING GUIDE
				Funnels, Swabs, Etc.	CD = Compact Dry DBDM = Brett (Stab Vida)	Sartorius Ampulized Media Or Nutrient Pad Sets
1	Storage & Filling Tanks Hoses & Piping	Wine Spoilage Microbes Fecal Microbes	Membrane Filtration (small vol.) Swab surfaces	(1) Biosart Monitor 100 Or (2) Biosart 250 Funnel & CD Sterile Swabs	CD Total Counts CD Yeast & Mold (Brett) DBDM Agar/broth	Standard Wort & Possibly (Acid tolerant) Orange Serum
2	Empty Bottles Rinse Water	Yeasts Fecal Microbes	Membrane Filtration Rinse Bottles – Sterile Water Rinse Water		CD Total Counts CD Yeast & Mold	Standard
3	Mouth of Bottles after filling	Yeasts	Swab		CD Yeast & Mold	Wort
4	Filling Machine Prefilter inlet/outlet Final filter outlet Corking Machine	Wine Spoilage Microbes	Swab filler bells Membrane Filtration Membrane Filtration Swab filler bells		CD Total Counts CD Yeast & Mold (Brett) DBDM Agar/broth	(Acid tolerant) Orange Serum Tomato Juice (Mold) Wort
5	Corks	Molds	Flush - Sterile Water Membrane Filter this water		CD Yeast & Mold	Wort
6	Filled bottles	Wine Spoilage Microbes	Membrane Filtration		CD Total Counts CD Yeast & Mold (Brett) DBDM Agar/broth	(Acid tolerant) Orange Serum Tomato Juice (Mold) Wort